Oliver Masucci was born in Stuttgart in 1968. From 1990 until 1994, he studied acting at the Hochschule der Künste in Berlin, and subsequently performed at theatres in Germany, Austria and Switzerland, including stints at the Schauspielhaus Basel (1995), the Deutsches Schauspielhaus in Hamburg (1996-2002), the Schauspiel Hannover (2000-2005) and the Schauspielhaus Zurich (2005-2009). His impressive dramatic range became visible in his lead performances in Tennessee Williams' "A Streetcar named Desire" (2007), George Bernard Shaw's "Major Barbara" (2009) and Heinrich von Kleist's "Amphitryon" (2009).

In addition to his stage career, Masucci began to appear in TV productions from the mid-1990s on. After roles in the miniseries "Die Manns – Ein Jahrhundertroman" ("The Manns - Novel of a Century", 2001, TV) and the theatrical release "Madrid" (2003), he got wider recognition with his turns in the two-part adventure film "Das Blut der Templer" ("Blood of the Templars", 2004, TV), the comedy "Zwei zum Fressen gern" ("Crocodile Alert", 2006, TV) and the disaster movie "Vulkan" ("Volcano", 2009, TV). Moreover, he guest-starred in several TV series including "SK Kölsch", "Soko Köln" and "Alarm für Cobra 11".

After joining the renowned Burgtheater in Vienna in 2009, Oliver Masucci dialed back on his TV and film engagements. On stage, he starred in productions of Shakespeare's "As You Like It", "Solaris" and "Marquise von O.".

In 2015, Masucci returned to the screen with lead roles in the TV production "Berlin Eins" and the adaptation of the bestselling novel "Er ist wieder da" ("Look Who's Back"). The latter saw him playing Adolf Hitler, who awakes in present-day Berlin and becomes a media celebrity. For this role, Oliver Masucci received a nomination as best actor in a lead role at the Deutscher Filmpreis 2016. In the same year, he appeared in a number of high-quality TV productions, e.g. playing an editor in chief in the political thriller "Die vierte Gewalt", "ugly Joe" in the three-part remake of the German classic "Winnetou" and a lobbyist in the thriller "Tödliche Geheimnisse". He also starred in the two critically-acclaimed series "4 Blocks" and "Dark" (both 2017).

He then played alongside Frederick Lau and Antje Traue in the feature film "Spielmacher" ("Playmaker", 2018) as well as in "Werk ohne Autor" (2018), the latter about a writer in the GDR directed by Oscar winning Florian Henckel von Donnersmarck. For a lead role in Oskar Roehlers satire "HERRliche Zeiten" ("Subs"), Masucci received another Deutscher Filmpreis nomination in 2018.

Afterwards Masucci was part of the ensemble of the award-winning TV production "Preis der Freiheit" (2019), which was set in the GDR; he also played the father of a daughter addicted to computer games in the highly acclaimed TV drama "Play" (2019).   

After a leading role as a Jewish family man during the Nazi era in "Als Hitler das rosa Kaninchen stahl" (DE/CH 2019, director: Caroline Link), Masucci worked with Oskar Roehler again: In "Enfant Terrible" (2020) he played the leading role of the legendary filmmaker, actor and author Rainer Werner Fassbinder.